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Journal of Clinical Microbiology, May 2005, p. 2303-2306, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2303-2306.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Korea

Kwan Soo Ko,1 Jin Yang Baek,1 Ji-Young Lee,1 Won Sup Oh,2 Kyong Ran Peck,2 NamYong Lee,3 Wee Gyo Lee,4 Kyungwon Lee,5 and Jae-Hoon Song1,2*

Asian-Pacific Research Foundation for Infectious Diseases, Seoul, Korea,1 Division of Infectious Diseases,2 Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea,3 Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea,4 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea5

Received 3 August 2004/ Returned for modification 8 November 2004/ Accepted 22 December 2004

A total of 98 vancomycin-resistant Enterococcus faecium (VREF) isolates from four tertiary-care hospitals in Korea during the period between 1998 and 2004 were analyzed for genotypic characteristics using the multiplex PCR, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and esp gene analysis. Ninety-two isolates of VREF with VanA phenotype and five of six isolates with VanB phenotype possessed the vanA gene. MLST analysis revealed 9 sequence types (STs), which belonged to a single clonal complex (CC78, clonal lineage C1). Five strains showing incongruence between phenotype and genotype (VanB-vanA) did not belong to the same genotypic clone. The esp gene was detected in all VREF strains, showing 12 different esp repeat profiles. Data suggest that an epidemic clonal group of VREF, CC78 with esp gene, is also present in Asia and has differentiated into multiple diverse genotypic clones during the evolutionary process.

* Corresponding author. Mailing address: Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Asian-Pacific Research Foundation for Infectious Diseases (ARFID), 50 Il-won dong, Kangnam-ku, Seoul 135-710, Korea. Phone: 82-2-3410-0320. Fax: 82-2-3410-0328. E-mail: jhsong{at}ansorp.org.

Journal of Clinical Microbiology, May 2005, p. 2303-2306, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2303-2306.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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