This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, B.
Right arrow Articles by Saksena, N. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, B.
Right arrow Articles by Saksena, N. K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2005, p. 2339-2344, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2339-2344.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

Bin Wang,1* Simon J. Potter,1 Yiguang Lin,2 Anthony L. Cunningham,1 Dominic E. Dwyer,1,3 Yuelong Su,4 Xuejun Ma,4 Yunde Hou,4 and Nitin K. Saksena1

Centre for Virus Research, Westmead Millennium Institute, The University of Sydney,1 Department of Health Sciences, University of Technology,2 CIDMLS, ICPMR, Westmead Hospital, Sydney, New South Wales, Australia,3 Beijing JDK Bio-technology Institute, National Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China4

Received 28 October 2004/ Returned for modification 17 November 2004/ Accepted 5 January 2005

The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (n = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.

* Corresponding author. Mailing address: Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute, The University of Sydney, Darcy Rd., Westmead, Sydney, NSW 2145, Australia. Phone: 61-2-98459107. Fax: 61-2-98459103. E-mail: bin_wang{at}wmi.usyd.edu.au.

Journal of Clinical Microbiology, May 2005, p. 2339-2344, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2339-2344.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhou, X., Kong, F., Sorrell, T. C., Wang, H., Duan, Y., Chen, S. C. A. (2008). Practical Method for Detection and Identification of Candida, Aspergillus, and Scedosporium spp. by Use of Rolling-Circle Amplification. J. Clin. Microbiol. 46: 2423-2427 [Abstract] [Full Text]  
  • Kong, F., Tong, Z., Chen, X., Sorrell, T., Wang, B., Wu, Q., Ellis, D., Chen, S. (2008). Rapid Identification and Differentiation of Trichophyton Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling-Circle Amplification. J. Clin. Microbiol. 46: 1192-1199 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»