This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, I-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, I-J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2005, p. 2444-2448, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2444-2448.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Immunofluorescence Assay for Detection of the Nucleocapsid Antigen of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Cells Derived from Throat Wash Samples of Patients with SARS

I-Jung Liu,1,2 Pei-Jer Chen,3 Shiou-Hwei Yeh,4 Yu-Ping Chiang,1 Li-Min Huang,5 Ming-Fu Chang,6 Shey-Ying Chen,7 Pan-Chyr Yang,8 Shan-Chwen Chang,8 Wei-Kung Wang,1,8* and the SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital

Institute of Microbiology,1 Clinical Medicine,3 Biochemistry and Molecular Biology, College of Medicine, National Taiwan University,6 Department of Pediatrics,5 Emergency Medicine,7 Internal Medicine, National Taiwan University Hospital,8 Cardinal Tien College of Nursing,2 National Health Research Institute, Taipei, Taiwan4

Received 7 September 2004/ Returned for modification 2 November 2004/ Accepted 6 December 2004

An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.

* Corresponding author. Mailing address: Insititute of Microbiology, College of Medicine, National Taiwan University, No. 1, Section 1, Jen-Ai Rd., Taipei, Taiwan. Phone: 8862-2312-3456, ext. 8286. Fax: 8862-2391-5293. E-mail: wwang60{at}yahoo.com.

Journal of Clinical Microbiology, May 2005, p. 2444-2448, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2444-2448.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mahony, J. B. (2008). Detection of Respiratory Viruses by Molecular Methods. Clin. Microbiol. Rev. 21: 716-747 [Abstract] [Full Text]  
  • Cheng, V. C. C., Lau, S. K. P., Woo, P. C. Y., Yuen, K. Y. (2007). Severe Acute Respiratory Syndrome Coronavirus as an Agent of Emerging and Reemerging Infection. Clin. Microbiol. Rev. 20: 660-694 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»