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Journal of Clinical Microbiology, May 2005, p. 2462-2464, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2462-2464.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR

Stéphanie Herwegh,1 Christophe Carnoy,2,3 Frédéric Wallet,1 Caroline Loïez,1 and René J. Courcol1*

Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire, Lille, France,1 INSERM E0364, Institut de Biologie de Lille, 1 rue du Pr. Calmette, 59021 Lille Cedex, France,2 Faculté des Sciences Pharmaceutiques et Biologiques, Lille, France3

Received 18 August 2004/ Returned for modification 19 August 2004/ Accepted 20 December 2004

An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Hygiène, Hôpital A. Calmette, F-59037-Lille Cedex, France. Phone: 33-320.444.908. Fax: 33-320.444.895. E-mail: rcourcol{at}chru-lille.fr.

Journal of Clinical Microbiology, May 2005, p. 2462-2464, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2462-2464.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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