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Journal of Clinical Microbiology, May 2005, p. 2465-2466, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2465-2466.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of a Commercial Reagent Leads to Reduced Germ Tube Production by Candida dubliniensis

Leigh E. Davis, Christine E. Shields, and William G. Merz*

Microbiology Division, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287-7093

Received 16 November 2004/ Returned for modification 18 December 2004/ Accepted 17 January 2005

The goal of this study was to determine the factor(s) explaining our inability to detect Candida dubliniensis. When germ tube-positive yeasts were tested for C. dubliniensis, no C. dubliniensis was detected; however, 58 C. dubliniensis strains were detected when germ tube-negative Candida albicans strains were tested further. Since all 58 C. dubliniensis strains detected were germ tube negative, these data implied that false-negative germ tube tests occurred with germ tube solution (GTS; Remel, Lenexa, KS). All 41 known C. dubliniensis strains tested were negative with GTS, whereas 40 were positive with rabbit serum (RS; Sigma-Aldrich, St. Louis, MO). Results for C. albicans were equivalent in GTS and RS. In conclusion, GTS cannot be used for the detection of C. dubliniensis, and switching from yeast to hyphae in C. dubliniensis is more restricted than in C. albicans.

* Corresponding author. Mailing address: Microbiology Laboratory, Department of Pathology, Meyer B1-193, The Johns Hopkins Hospital, Baltimore, MD 21287-7093. Phone: (410) 955-5077. Fax: (410) 614-8087. E-mail: wmerz{at}jhmi.edu.

Journal of Clinical Microbiology, May 2005, p. 2465-2466, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2465-2466.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gherna, M., Merz, W. G. (2009). Identification of Candida albicans and Candida glabrata within 1.5 Hours Directly from Positive Blood Culture Bottles with a Shortened Peptide Nucleic Acid Fluorescence In Situ Hybridization Protocol. J. Clin. Microbiol. 47: 247-248 [Abstract] [Full Text]  


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