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Journal of Clinical Microbiology, June 2005, p. 2586-2589, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2586-2589.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Flow Cytometry Susceptibility Testing for the Antifungal Caspofungin

M. Mitchell,1,2* M. Hudspeth,2 and A. Wright2

Microbiology Department, Community Medical Centers—Fresno, Fresno, California 93701,1 Department of Biology, California State University, Fresno, California 937202

Received 12 November 2004/ Returned for modification 16 December 2004/ Accepted 16 February 2005

Rapid antifungal susceptibility testing for the antifungal agent caspofungin can be performed using flow cytometry (FC). An FC procedure using acridine orange provided minimum inhibitory concentration (MIC) results within 7 to 9 h which were compared with results obtained using the NCCLS M27-A2 protocol. To evaluate the consistency of this method, susceptibility testing using caspofungin was performed using 73 isolates of eight different species of Candida from various clinical samples in Central California. Macrotiter or microdilution tests were performed according to the NCCLS M27-A2 protocol, and the MICs were compared to those provided by our flow cytometry method. All isolates tested had results within the sensitive interpretive category, and 90% of the results compared within 1 dilution, showing excellent agreement between the methods. The MIC at which 50% of the isolates tested were inhibited (MIC50) and the MIC90 of caspofungin for all eight Candida species were within 1 dilution. This flow cytometer 7-h protocol for testing the antifungal susceptibility of Candida species to caspofungin provided results equivalent to those obtained with the M27-A2 protocol.

* Corresponding author. Mailing address: Microbiology Department, Community Medical Centers—Fresno, Fresno, CA 93701. Phone: (559) 459-2021. Fax: (559) 459-6542. E-mail: mmitchell{at}communitymedical.org.

Journal of Clinical Microbiology, June 2005, p. 2586-2589, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2586-2589.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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