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Selection of Strains for Quality Assessment of the Disk Induction Method for Detection of Inducible Clindamycin Resistance in Staphylococci: a CLSI Collaborative Study

Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892,¹ Microbiology Laboratory, Clinical Microbiology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114,² Acute Disease Epidemiology, Minnesota Department of Health, Minneapolis, Minnesota 55414,³ Department of Pathology and Laboratory Medicine, University of California at Los Angeles Medical Center, Los Angeles, California 90025,⁴ Clinical Microbiology Laboratory, Baylor University Medical Center, Dallas, Texas 75246,⁵ Clinical Microbiology Laboratory, Stanford University Medical School, Stanford, California 94305,⁶ Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710,⁷ Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,⁸ Department of Pathology, University of Texas Health Science Center, San Antonio, Texas 78229⁹

Received 3 December 2004/ Returned for modification 5 January 2005/ Accepted 14 February 2005

A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four strains of Staphylococcus aureus were tested as unknowns on 10 different days in each laboratory using the recently recommended CLSI (formerly NCCLS) disk diffusion method and the inoculum purity control method. Strains contained either macrolide-lincosamide-streptogramin B (MLS_B) resistance genes encoded by erm(A) or erm(C) or a macrolide resistance efflux pump encoded by msr(A). Based upon the results of this study, strain UT 32 (now designated ATCC strain BAA-977) containing erm(A) is recommended as the positive control organism for inducible clindamycin resistance. Strain UT 25 (now designated ATCC BAA-976), which harbors the efflux pump encoded by msr(A), is recommended as the negative control organism.

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