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Journal of Clinical Microbiology, June 2005, p. 2771-2781, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2771-2781.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Campylobacter jejuni Lipooligosaccharide Biosynthesis Loci from a Variety of Sources

Craig T. Parker,1* Sharon T. Horn,1 Michel Gilbert,2 William G. Miller,1 David L. Woodward,3 and Robert E. Mandrell1

Produce Safety and Microbiology Unit, WRRC, USDA, ARS, 800 Buchanan St., Albany, California 94710,1 Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Dr., Ottawa, Ontario K1A 0R6,2 Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Winnipeg, Canada3

Received 28 October 2004/ Returned for modification 29 December 2004/ Accepted 15 February 2005

Campylobacter jejuni strains exhibit significant variation in the genetic content of the lipooligosaccharide (LOS) biosynthesis loci with concomitant differences in LOS structure. The C. jejuni LOS loci have been grouped into six classes based on gene content and organization. Utilizing PCR amplifications of genes from these loci, we were able to classify a majority (80%) of the LOS biosynthesis loci from 123 strains of C. jejuni that included 39 of the Penner serotype reference strains. We found that a particular LOS class was not always associated with a specific Penner serotype, and 14 of 16 Guillain-Barré syndrome-associated isolates tested in this study shared the same LOS class. The remaining isolates that could not be classified were often distinguishable from each other based on the results of gene-specific PCR and the lengths of their LOS biosynthesis loci as determined by long (XL) PCR. Sequence analysis of two of these unique XL PCR products demonstrated two new LOS classes. These results support the hypothesis that the LOS locus is a hot spot for genetic exchange and rearrangements. Analysis of the LOS biosynthesis genes by PCR assays can be used for typing C. jejuni and offers the advantage of inferring potential LOS structures.

* Corresponding author. Mailing address: Produce Safety and Microbiology Unit, WRRC, USDA, ARS, 800 Buchanan St., Albany, CA 94710. Phone: (510) 559-6187. Fax: (510) 559-6162. E-mail: parker{at}pw.usda.gov.

Journal of Clinical Microbiology, June 2005, p. 2771-2781, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2771-2781.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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