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Journal of Clinical Microbiology, June 2005, p. 2904-2908, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2904-2908.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Immunohistochemistry and Virus Isolation for Diagnosis of West Nile Virus

Angela E. Ellis,1* Daniel G. Mead,3 Andrew B. Allison,3 Samantha E. J. Gibbs,3 Nicole L. Gottdenker,4 David E. Stallknecht,2 and Elizabeth W. Howerth1

Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia,1 Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens, Georgia,2 Southeastern Cooperative Wildlife Disease Study, Athens, Georgia,3 Institute of Ecology, University of Georgia, Athens, Georgia4

Received 22 September 2004/ Returned for modification 19 October 2004/ Accepted 7 January 2005

Immunohistochemistry and virus isolation were performed on 1,057 birds. Immunohistochemistry, virus isolation, or both found 325 birds to be West Nile virus positive. Of these, 271 were positive by both methods. These results indicate that virus isolation and immunohistochemistry are approximately equal in their ability to detect West Nile virus.

* Corresponding author. Mailing address: Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602. Phone: (706) 583-0476. Fax: (706) 542-5828. E-mail: aellis{at}vet.uga.edu.

Journal of Clinical Microbiology, June 2005, p. 2904-2908, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2904-2908.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nemeth, N. M., Young, G. R., Burkhalter, K. L., Brault, A. C., Reisen, W. K., Komar, N. (2009). West Nile virus detection in nonvascular feathers from avian carcasses. jvdi 21: 616-622 [Abstract] [Full Text]  
  • Ellis, A. E., Mead, D. G., Allison, A. B., Stallknecht, D. E., Howerth, E. W. (2007). PATHOLOGY AND EPIDEMIOLOGY OF NATURAL WEST NILE VIRAL INFECTION OF RAPTORS IN GEORGIA. J Wildl Dis 43: 214-223 [Abstract] [Full Text]  


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