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Journal of Clinical Microbiology, June 2005, p. 2996-2997, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2996-2997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Extraction of Mycobacterium tuberculosis DNA: a Question of Containment

Wendy Somerville,1 Louise Thibert,2 Kevin Schwartzman,3,4 and Marcel A. Behr1,3*

McGill University Health Centre Research Institute,1 Institut National de Santé Publique du Québec,2 Department of Medicine, McGill University Health Centre,3 Respiratory Epidemiology Unit, McGill University, Montréal, Québec, Canada4

Received 29 December 2004/ Returned for modification 3 February 2005/ Accepted 23 February 2005

DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.

* Corresponding author. Mailing address: Division of Infectious Diseases and Microbiology, A5.156, Montreal General Hospital, 1650 Cedar Ave., Montreal, H3G 1A4 Quebec, Canada. Phone: (514) 934-1934, ext. 42815. Fax: (514) 934-8423. E-mail: marcel.behr{at}mcgill.ca.

Journal of Clinical Microbiology, June 2005, p. 2996-2997, Vol. 43, No. 6
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.6.2996-2997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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