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Journal of Clinical Microbiology, July 2005, p. 3213-3220, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3213-3220.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of a Shiga Toxin-, Intimin-, and Enterotoxin Hemolysin-Producing Escherichia coli ONT:H25 Strain Commonly Isolated from Healthy Cattle

Haiqing Sheng,1 Margaret A. Davis,1 Hannah J. Knecht,1 Dale D. Hancock,2 Joyce Van Donkersgoed,3 and Carolyn J. Hovde1*

University of Idaho, Department of Microbiology, Molecular Biology, and Biochemistry, Moscow, Idaho 83844-3052,1 Washington State University, Department of Veterinary Medicine, Pullman, Washington,2 Veterinary Services Inc., Lacombe, Alberta, Canada3

Received 13 January 2005/ Returned for modification 27 February 2005/ Accepted 27 March 2005

Among bovine fecal and recto-anal mucosal swab samples cultured in our laboratory for Escherichia coli O157:H7, we frequently isolated E. coli organisms that were phenotypically similar to the O157:H7 serotype as non-sorbitol fermenting and negative for ß-glucuronidase activity but serotyped O nontypeable:H25 (ONT:H25). This study determined the prevalence and virulence properties of the E. coli ONT:H25 isolates. Among dairy and feedlot cattle (n = 170) sampled in Washington, Idaho, and Alberta, Canada, the percentage of animals culture positive for E. coli ONT:H25 ranged from 7.5% to 22.5%, compared to the prevalence of E. coli O157:H7 that ranged from 0% to 15%. A longitudinal 8-month study of dairy heifers (n = 40) showed that 0 to 15% of the heifers were culture positive for E. coli O157:H7, while 15 to 22.5% of the animals were culture positive for E. coli ONT:H25. As determined by a multiplex PCR, the E. coli ONT:H25 isolates carried a combination of virulence genes characteristic of the enterohemorrhagic E. coli, including intimin, translocated intimin receptor, Stx2, and hemolysin (eae-ß, tir, stx2vh-a, and hly). E. coli ONT:H25 isolates from diverse geographic locations and over time were fingerprinted by separating XbaI-restricted chromosomal DNA by pulsed-field gel electrophoresis (PFGE) separation. Two strains of E. coli ONT:H25 were highly similar by PFGE pattern. Experimental inoculation of cattle showed that E. coli ONT:H25, like E. coli O157:H7, colonized the bovine recto-anal junction mucosa for more than 4 weeks following a single rectal application of bacteria.

* Corresponding author. Mailing address: Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844-3052. Phone: (208) 885-5906. Fax: (208) 885-6518. E-mail: cbohach{at}uidaho.edu.

Journal of Clinical Microbiology, July 2005, p. 3213-3220, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3213-3220.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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