JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Francois, P.
Right arrow Articles by Schrenzel, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Francois, P.
Right arrow Articles by Schrenzel, J.
Journal of Clinical Microbiology, July 2005, p. 3346-3355, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3346-3355.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of an Automated Multiple-Locus, Variable-Number Tandem Repeat-Based Method for Rapid and High-Throughput Genotyping of Staphylococcus aureus Isolates

Patrice Francois,1* Antoine Huyghe,1 Yvan Charbonnier,1 Manuela Bento,1 Sébastien Herzig,2 Ivan Topolski,1 Bénédicte Fleury,3 Daniel Lew,3 Pierre Vaudaux,3 Stephan Harbarth,3,4 Willem van Leeuwen,5 Alex van Belkum,5 Dominique S. Blanc,6 Didier Pittet,3,4 and Jacques Schrenzel1,2

Genomic Research Laboratory,1 Clinical Microbiology Laboratory,2 Service of Infectious Diseases,3 Infection Control Program, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland,4 Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands,5 Hospital of Preventive Medicine, University Hospital of Lausanne, CH-1011 Lausanne, Switzerland6

Received 15 September 2004/ Returned for modification 5 November 2004/ Accepted 14 March 2005

Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for its discriminatory power and reproducibility with clinical isolates of various origins, including a panel of control strains previously characterized by several typing methods and collections from either long-term carriers or defined nosocomial outbreaks. All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time.

* Corresponding author. Mailing address: University of Geneva Hospitals, Service of Infectious Diseases/Genomic Research Laboratory, CH-1211 Geneva 14, Switzerland. Phone: (41)-22 3729338. Fax: (41)-22 3729830. E-mail:patrice.francois{at}genomic.ch.

Journal of Clinical Microbiology, July 2005, p. 3346-3355, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3346-3355.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2005 by the American Society for Microbiology. All rights reserved.