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Journal of Clinical Microbiology, July 2005, p. 3427-3430, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3427-3430.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development of a Novel Protein Microarray Method for Serotyping Salmonella enterica Strains

H. Y. Cai,1,3 L. Lu,1 C. A. Muckle,2 J. F. Prescott,3 and S. Chen1*

Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada,1 OIE Reference Laboratory for Salmonellosis, Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada,2 Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada3

Received 28 February 2005/ Accepted 15 March 2005

An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allowed complete serovar identification of 86 target strains and partial identification of 30 target strains and allowed exclusion of the 73 nontarget strains from the target serovars.

* Corresponding author. Mailing address: University of Guelph, Laboratory Services, P.O. Box 3650, 95 Stone Road West, Guelph, Ontario, Canada N1H 8J7. Phone: 519-767-6319. Fax: 519-767-6240. E-mail: schen{at}lsd.uoguelph.ca.

Journal of Clinical Microbiology, July 2005, p. 3427-3430, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3427-3430.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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