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Clinical Use of 16S rRNA Gene Sequencing To Identify Mycoplasma felis and M. gateae Associated with Feline Ulcerative Keratitis

TriHealth Laboratories and Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine,¹ All Animal Eye Clinic, Cincinnati, Ohio,² Departments of Medicine and Pathology, Vanderbilt University, Nashville, Tennessee³

Received 25 February 2005/ Accepted 21 March 2005

Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less empirical and more organism-specific therapy.

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