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Journal of Clinical Microbiology, July 2005, p. 3438-3442, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3438-3442.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA

Shannon Kaplan,¹ Elizabeth M. Marlowe,^1* James J. Hogan,¹ Mehmet Doymaz,^2, David A. Bruckner,² and Andrew E. Simor³

Gen-Probe Incorporated, San Diego, California,¹ UCLA Medical Center, Department of Pathology and Laboratory Medicine, Los Angeles, California,² Sunnybrook and Women's College Health Sciences Centre, Department of Microbiology, Toronto, Canada³

Received 29 November 2004/ Returned for modification 24 January 2005/ Accepted 21 March 2005

rRNA gene sequences were used for identification and target adequacy controls in a DNA probe assay to identify isolates as Staphylococcus and, more specifically, as S. aureus within 1 hour. mecA status was simultaneously determined using a specific DNA probe. The target adequacy control guarded against false-negative mecA results.

Present address: Beth Israel Medical Center, New York, N.Y.