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Journal of Clinical Microbiology, July 2005, p. 3443-3446, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3443-3446.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Human Metapneumovirus Strains in Nasopharyngeal Aspirates and Shell Vial Cultures by Monoclonal Antibodies

Servizio di Virologia, IRCCS Policlinico San Matteo,¹ Istituto di Chimica Biologica, Università di Pavia, 27100 Pavia, Italy²

Received 14 January 2005/ Returned for modification 7 February 2005/ Accepted 15 February 2005

Monoclonal antibodies to human metapneumovirus (hMPV) were developed for direct fluorescent antibody (DFA) staining of nasopharyngeal aspirates from 40 infants with respiratory infections, as well as for hMPV identification in shell vial cultures. With reference to reverse transcription-PCR, DFA staining showed sensitivity, specificity, and positive and negative predictive values of 73.9%, 94.1%, 94.4%, and 72.7%, respectively. Monoclonal antibodies are useful for direct hMPV detection.

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