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Journal of Clinical Microbiology, July 2005, p. 3450-3452, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3450-3452.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Conventional Susceptibility Testing, Penicillin-Binding Protein 2a Latex Agglutination Testing, and mecA Real-Time PCR for Detection of Oxacillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococcus

Melissa B. Miller,1,2* Heather Meyer,1 Elaine Rogers,1 and Peter H. Gilligan1,2,3

University of North Carolina Hospitals and University of North Carolina School of Medicine,1 Departments of Pathology and Laboratory Medicine,2 Microbiology, Chapel Hill, North Carolina3

Received 19 November 2004/ Returned for modification 24 January 2005/ Accepted 28 March 2005

Penicillin-binding protein (PBP) 2a latex agglutination was compared with conventional susceptibility testing and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus. Inoculum volume and induction with oxacillin were PBP 2a testing variables. For coagulase-negative Staphylococcus, an increased inoculum volume of 10 µl greatly reduced the number of isolates requiring induction.

* Corresponding author. Mailing address: University of North Carolina School of Medicine, Campus Box 7525, Chapel Hill, NC 27599-7525. Phone: (919) 966-3723. Fax: (919) 966-0486. E-mail: mbmiller{at}unch.unc.edu.

Journal of Clinical Microbiology, July 2005, p. 3450-3452, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3450-3452.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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