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Journal of Clinical Microbiology, July 2005, p. 3463-3466, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3463-3466.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of PCR Analyses To Define the Distribution of Ralstonia Species Recovered from Patients with Cystic Fibrosis

Laboratory for Microbiology, Faculty of Sciences, University of Ghent, Ghent, Belgium,¹ Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109²

Received 24 February 2005/ Accepted 1 April 2005

Two new PCR assays (for Ralstonia species and Ralstonia respiraculi), together with previously published PCR assays, were used to assess Ralstonia isolates recovered from 111 cystic fibrosis patients. Ralstonia mannitolilytica accounted for 46% of isolates, while R. respiraculi and Ralstonia pickettii accounted for 19% and 18%, respectively. Ralstonia basilensis and Ralstonia metallidurans, species not previously recovered from human samples, were also identified.

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