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Journal of Clinical Microbiology, July 2005, p. 3487-3490, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3487-3490.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Identification of Seven Streptococcus pneumoniae Serotypes Targeted by a 7-Valent Conjugate Vaccine

Damien M. O'Halloran1 and Mary T. Cafferkey1,2*

Epidemiology and Molecular Biology Unit, The Children's University Hospital,1 Department of Clinical Microbiology, Royal College of Surgeons in Ireland, York Street, Dublin, Ireland2

Received 9 December 2004/ Returned for modification 31 January 2005/ Accepted 12 February 2005

Here we describe a Streptococcus pneumoniae one-step multiplex PCR assay which identifies by amplicon size the seven capsular polysaccharide serotypes targeted by the 7-valent conjugate vaccine. The multiplex PCR assay was used to blindly assay clinical isolates recovered during 1999 in the Republic of Ireland from cases of invasive disease whose serotypes were previously determined by classical methods.

* Corresponding author. Mailing address: The Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland. Phone: IAC-353-1-878-4858. Fax: IAC-353-1-878-4856. E-mail: M.Cafferkey{at}tsch.ie.

Journal of Clinical Microbiology, July 2005, p. 3487-3490, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3487-3490.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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