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Journal of Clinical Microbiology, July 2005, p. 3494-3496, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3494-3496.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of seaFAST, a Rapid Fluorescent In Situ Hybridization Test, for Detection of Helicobacter pylori and Resistance to Clarithromycin in Paraffin-Embedded Biopsy Sections

Julie M. Morris,1* Alisa L. Reasonover,1 Michael G. Bruce,1 Dana L. Bruden,1 Brian J. McMahon,1,2 Frank D. Sacco,2 Douglas E. Berg,3 and Alan J. Parkinson1

Arctic Investigations Program, Centers for Disease Control and Prevention, Anchorage, Alaska,1 Alaska Native Tribal Health Consortium, Anchorage, Alaska,2 Washington University School of Medicine, St. Louis, Missouri3

Received 4 January 2004/ Returned for modification 9 March 2005/ Accepted 25 March 2005

A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detection of H. pylori and comparable to agar dilution for the detection of resistance to clarithromycin.

* Corresponding author. Mailing address: Arctic Investigations Program, CDC, 4055 Tudor Centre Drive, Anchorage, AK 99508. Phone: (907) 729-3445. Fax: (907) 729-3429. E-mail: jmorris{at}cdc.gov.

Journal of Clinical Microbiology, July 2005, p. 3494-3496, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3494-3496.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Noguchi, N., Rimbara, E., Kato, A., Tanaka, A., Tokunaga, K., Kawai, T., Takahashi, S., Sasatsu, M. (2007). Detection of mixed clarithromycin-resistant and -susceptible Helicobacter pylori using nested PCR and direct sequencing of DNA extracted from faeces. J Med Microbiol 56: 1174-1180 [Abstract] [Full Text]  


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