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Journal of Clinical Microbiology, July 2005, p. 3497-3499, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3497-3499.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Disk Diffusion Testing Using Candida sp. Colonies Taken Directly from CHROMagar Candida Medium May Decrease Time Required To Obtain Results

Michael Klevay,1 Alex Ebinger,2 Daniel Diekema,1,2,4* Shawn Messer,2 Richard Hollis,2 and Michael Pfaller2,3

Departments of Internal Medicine,1 Pathology,University of Iowa Carver College of Medicine,2 Department of Epidemiology, University of Iowa College of Public Health,3 Iowa City Veterans Affairs Medical Center, Iowa City, Iowa4

Received 11 November 2004/ Returned for modification 25 December 2004/ Accepted 14 March 2005

We compared results of disk diffusion antifungal susceptibility testing from Candida sp. strains passaged on CHROMagar and on potato dextrose agar. The overall categorical agreements for fluconazole and voriconazole disk testing were 95% and 98% with 0% and 0.5% very major errors, respectively. Disk diffusion testing by the CLSI (formerly NCCLS) M44-A method can be performed accurately by taking inocula directly from CHROMagar.

* Corresponding author. Mailing address: C 606 General Hospital, University of Iowa Hospital, 200 Hawkins Drive, Iowa City, Iowa 52246. Phone: (319) 356-8615. Fax: (319) 356-4916. E-mail: daniel-diekema{at}uiowa.edu.

Journal of Clinical Microbiology, July 2005, p. 3497-3499, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3497-3499.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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