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Journal of Clinical Microbiology, July 2005, p. 3500-3503, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3500-3503.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Intra- and Interpatient Variability of the hsp65 and 16S-23S Intergenic Gene Region in Mycobacterium abscessus Strains from Patients with Cystic Fibrosis

Brigitte König,1* Ina Tammer,1 Veronika Sollich,2 and Wolfgang König1

Institute of Medical Microbiology,1 Department of Pediatrics, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany2

Received 19 August 2004/ Returned for modification 14 November 2004/ Accepted 10 January 2005

To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic sequence (ITS). We detected two different hsp65 genes and correspondingly two ITS sequevars belonging to M. abscessus type I and type II.

* Corresponding author. Mailing address: Otto-von-Guericke University Magdeburg, Institute of Medical Microbiology, Leipzigerstr. 44, D-39120 Magdeburg, Germany. Phone: 49 391 6713353. Fax: 49 391 6713384. E-mail: brigitte.koenig{at}medizin.uni-magdeburg.de.

Journal of Clinical Microbiology, July 2005, p. 3500-3503, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3500-3503.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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