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Journal of Clinical Microbiology, July 2005, p. 3504-3507, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3504-3507.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of the COBAS TaqMan 48 Real-Time PCR System for Quantitation of Hepatitis B Virus DNA

Rafael M. Gordillo, Juan Gutiérrez, and Manuel Casal*

Microbiology and Parasitology Department, Hospital Universitario Reina Sofía, Cordoba, Spain

Received 24 August 2004/ Returned for modification 14 January 2005/ Accepted 22 February 2005

The purpose of this study is to evaluate the usefulness of the new real-time PCR COBAS TaqMan 48 analyzer, comparing it to the existing COBAS AMPLICOR HBV MONITOR based on conventional PCR technology. The study used 104 samples from different patients. No differences were found in the sensitivity of the tests. There was an excellent correlation between the sample with a viral load within the dynamic range of the two tests (r = 0.938). The COBAS TaqMan test has a wider linear range, and this fact enables quantifying of the viral load without diluting the sample.

* Corresponding author. Mailing address: Hospital Universitario Reina Sofía, Avda. Menéndez Pidal s/n, 14004 Cordoba, Spain. Phone: 34 957 218284. Fax: 34 957 218239. E-mail: mi1carom{at}uco.es.

Journal of Clinical Microbiology, July 2005, p. 3504-3507, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3504-3507.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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