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Journal of Clinical Microbiology, July 2005, p. 3516-3518, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3516-3518.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Tropheryma whipplei DNA in Clinical Specimens by LightCycler Real-Time PCR

Lynne M. Sloan, Jon E. Rosenblatt, and Franklin R. Cockerill III*

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota

Received 18 October 2004/ Returned for modification 6 December 2004/ Accepted 24 February 2005

A real-time PCR method using the LightCycler (Roche Applied Science, Indianapolis, IN) was compared to a conventional PCR assay for the detection of Tropheryma whipplei in 321 clinical specimens. The LightCycler method had sensitivity and specificity comparable to the conventional method but required considerably less labor and time (3 h versus 3 to 5 days).

* Corresponding author. Mailing address: Division of Clinical Microbiology, Department of Laboratory Medicine & Pathology, Mayo Clinic—Mayo Foundation, 200 First Street SW, Rochester, MN 55905. Phone: (507) 284-3697. Fax: (507) 284-4272. E-mail: cockerill.franklin{at}mayo.edu.

Journal of Clinical Microbiology, July 2005, p. 3516-3518, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3516-3518.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schneemann, M., Schoedon, G., Sloan, L. M., Rosenblatt, J. E., Cockerill, F. R. III (2005). Whipple's DNA Is Not Whipple's Disease. J. Clin. Microbiol. 43: 5414-5414 [Full Text]  


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