This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hsiao, C. R.
Right arrow Articles by Chang, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsiao, C. R.
Right arrow Articles by Chang, T. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2005, p. 3760-3768, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3760-3768.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Medically Important Molds by an Oligonucleotide Array{dagger}

Chen Ren Hsiao,1 Liyin Huang,1 Jean-Philippe Bouchara,2 Richard Barton,3 Hsin Chieh Li,1 and Tsung Chain Chang1*

Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China,1 Host-Parasite-Interaction Study Group (UPRES-EA 3142), Laboratory of Parasitology and Mycology, University Hospital, Angers, France,2 School of Biochemistry and Microbiology, University of Leeds, United Kingdom3

Received 22 February 2005/ Returned for modification 1 April 2005/ Accepted 2 May 2005

Infections caused by fungi have increased in recent years. Accurate and rapid identification of fungal pathogens is important for appropriate treatment with antifungal agents. On the basis of the internal transcribed spacer 1 (ITS 1) and ITS 2 sequences of the rRNA genes, an oligonucleotide array was developed to identify 64 species (32 genera) of clinically important filamentous (or dimorphic) fungi. These 64 species included fungi causing superficial, cutaneous, subcutaneous, and invasive infections. The method consisted of PCR amplification of the ITS regions using a pair of universal primers, followed by hybridization of the digoxigenin-labeled PCR products to a panel of species- or group-specific oligonucleotides immobilized on a nylon membrane. Of 397 fungal strains (290 target and 107 nontarget strains) tested, the sensitivity and specificity of the array was 98.3% (285/290) and 98.1% (105/107), respectively. Misidentified strains were usually those belonging to the same genus of the target species or having partial homology with oligonucleotide probes on the membrane. The whole procedure can be finished within 24 h starting from isolated colonies; reproductive structures, which are essential for the conventional identification methods, are not needed. In conclusion, the present array is a powerful tool for identification of clinically important filamentous fungi and may have the potential to be continually extended by adding further oligonucleotides to the array without significantly increasing the cost or complexity.

* Corresponding author. Mailing address: Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan, Republic of China. Phone: 886-6-2353535, ext. 5790. Fax: 886-6-2363956. E-mail: tsungcha{at}mail.ncku.edu.tw.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org.

Journal of Clinical Microbiology, August 2005, p. 3760-3768, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3760-3768.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miller, M. B., Tang, Y.-W. (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22: 611-633 [Abstract] [Full Text]  
  • Oechsler, R. A., Feilmeier, M. R., Ledee, D. R., Miller, D., Diaz, M. R., Fini, M. E., Fell, J. W., Alfonso, E. C. (2009). Utility of Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium spp. from Ocular Sources. IOVS 50: 2230-2236 [Abstract] [Full Text]  
  • Bouchara, J.-P., Hsieh, H. Y., Croquefer, S., Barton, R., Marchais, V., Pihet, M., Chang, T. C. (2009). Development of an Oligonucleotide Array for Direct Detection of Fungi in Sputum Samples from Patients with Cystic Fibrosis. J. Clin. Microbiol. 47: 142-152 [Abstract] [Full Text]  
  • Zhou, X., Kong, F., Sorrell, T. C., Wang, H., Duan, Y., Chen, S. C. A. (2008). Practical Method for Detection and Identification of Candida, Aspergillus, and Scedosporium spp. by Use of Rolling-Circle Amplification. J. Clin. Microbiol. 46: 2423-2427 [Abstract] [Full Text]  
  • Li, H. C., Bouchara, J.-P., Hsu, M. M.-L., Barton, R., Su, S., Chang, T. C. (2008). Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions. J Med Microbiol 57: 592-600 [Abstract] [Full Text]  
  • Spiess, B., Seifarth, W., Hummel, M., Frank, O., Fabarius, A., Zheng, C., Morz, H., Hehlmann, R., Buchheidt, D. (2007). DNA Microarray-Based Detection and Identification of Fungal Pathogens in Clinical Samples from Neutropenic Patients. J. Clin. Microbiol. 45: 3743-3753 [Abstract] [Full Text]  
  • Li, H. C., Bouchara, J.-P., Hsu, M. M.-L., Barton, R., Chang, T. C. (2007). Identification of Dermatophytes by an Oligonucleotide Array. J. Clin. Microbiol. 45: 3160-3166 [Abstract] [Full Text]  
  • Zeng, X., Kong, F., Halliday, C., Chen, S., Lau, A., Playford, G., Sorrell, T. C. (2007). Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens. J. Clin. Microbiol. 45: 2872-2880 [Abstract] [Full Text]  
  • Lau, A., Chen, S., Sorrell, T., Carter, D., Malik, R., Martin, P., Halliday, C. (2007). Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue Specimens. J. Clin. Microbiol. 45: 380-385 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»