This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, L.
Right arrow Articles by Jin, Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, L.
Right arrow Articles by Jin, Q.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2005, p. 3835-3839, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3835-3839.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic Characteristics of Human Enterovirus 71 and Coxsackievirus A16 Circulating from 1999 to 2004 in Shenzhen, People's Republic of China{dagger}

Linlin Li,1 Yaqing He,2 Hong Yang,2 Junping Zhu,1 Xingye Xu,1 Jie Dong,1 Yafang Zhu,1 and Qi Jin1*

State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese CDC, Beijing,1 Department of Microbiology, Shenzhen Center for Disease Control and Prevention, Shenzhen, People's Republic of China2

Received 2 February 2005/ Returned for modification 16 March 2005/ Accepted 16 May 2005

The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 stool specimens, 60 showed positive signals when screened with EV71- and CA16-specific primers. EV71 was identified in 19 specimens, and CA16 was identified in 41 specimens; coinfection by EV71 and CA16 was not observed. Phylogenetic analysis of all EV71 strains isolated from the mainland Chinese samples established C4 as the predominant genotype. Only one other known strain (3254-TAI-98; AF286531), isolated in Taiwan in 1998, was identified as belonging to genotype C4. Phylogenetic analysis of CA16 strains allowed us to identify three new genetic lineages (A, B, and C), with lineage C recently predominating in Asian countries, such as the People's Republic of China, Malaysia, and Japan. These new observations indicate that CA16 circulating in the People's Republic of China is genetically diverse, and additional surveillance is warranted.

* Corresponding author. Mailing address: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese CDC, No. 6 Rongjing East Street, Yi zhuang, Beijing 100176, China. Phone: 86 10 67876915. Fax: 86 10 67877736. E-mail: zdsys{at}vip.sina.com.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, August 2005, p. 3835-3839, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.3835-3839.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, S.-W., Hsu, Y.-W., Smith, D. J., Kiang, D., Tsai, H.-P., Lin, K.-H., Wang, S.-M., Liu, C.-C., Su, I.-J., Wang, J.-R. (2009). Reemergence of Enterovirus 71 in 2008 in Taiwan: Dynamics of Genetic and Antigenic Evolution from 1998 to 2008. J. Clin. Microbiol. 47: 3653-3662 [Abstract] [Full Text]  
  • van der Sanden, S., Koopmans, M., Uslu, G., van der Avoort, H., on behalf of the Dutch Working Group for Clinical, (2009). Epidemiology of Enterovirus 71 in The Netherlands, 1963 to 2008. J. Clin. Microbiol. 47: 2826-2833 [Abstract] [Full Text]  
  • Yang, F., Ren, L., Xiong, Z., Li, J., Xiao, Y., Zhao, R., He, Y., Bu, G., Zhou, S., Wang, J., Qi, J. (2009). Enterovirus 71 Outbreak in the People's Republic of China in 2008. J. Clin. Microbiol. 47: 2351-2352 [Full Text]  
  • Arita, M., Nagata, N., Iwata, N., Ami, Y., Suzaki, Y., Mizuta, K., Iwasaki, T., Sata, T., Wakita, T., Shimizu, H. (2007). An Attenuated Strain of Enterovirus 71 Belonging to Genotype A Showed a Broad Spectrum of Antigenicity with Attenuated Neurovirulence in Cynomolgus Monkeys. J. Virol. 81: 9386-9395 [Abstract] [Full Text]  
  • Hosoya, M., Kawasaki, Y., Sato, M., Honzumi, K., Hayashi, A., Hiroshima, T., Ishiko, H., Kato, K., Suzuki, H. (2007). Genetic Diversity of Coxsackievirus A16 Associated with Hand, Foot, and Mouth Disease Epidemics in Japan from 1983 to 2003. J. Clin. Microbiol. 45: 112-120 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»