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Journal of Clinical Microbiology, August 2005, p. 4022-4025, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4022-4025.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Value of Serological Testing for Diagnosis of Legionellosis in Outbreak Patients

Almudena Rojas,1 M. Dolores Navarro,2 Francisca E. Fornés,2 Estefanía Serra,2 Encarnación Simarro,2 José Rojas,1* and Joaquín Ruiz2

Vircell S. L., Pza. Domínguez Ortiz 1, 18320 Santa Fé, Granada, Spain,1 Laboratorio de Microbiología, Hospital Virgen de la Arrixaca, 30120 El Palmar, Murcia, Spain2

Received 4 November 2004/ Returned for modification 20 December 2004/ Accepted 20 May 2005

Serum antibody detection tests and a urine antigen detection technique were compared in samples from 116 patients epidemiologically characterized as belonging to a legionellosis outbreak. Sera were tested by enzyme-linked immunosorbent assays (ELISAs) for immunoglobulin M (IgM) and IgG plus IgM and by immunofluorescent assays (IFAs) for IgG, IgM, IgA, and polyimmunoglobulin using commercial kits (Vircell); concentrated urines were tested with the Binax NOW Legionella test. ELISA for IgM, ELISA for IgG plus IgM, antigenuria detection, and IFA for IgM were able to diagnose 72.3%, 60.5%, 53.3%, and 51.4%, respectively, of patients. Antigenuria was present in 53.8% of first samples, ELISA detected IgM in 29.7%, ELISA detected IgG plus IgM in 7.9%, and IFA detected IgM in 3.9%. Ten antigenuria-negative first samples tested serologically positive, 9 of them to IgM by ELISA. Despite the single source of the samples included in the study, detection of IgM using a sensitive technique such as ELISA seems to be a suitable complement to antigenuria detection for the diagnosis of legionellosis.

* Corresponding author. Mailing address: Vircell S. L., Plaza Domínguez Ortiz 1, Polígono Industrial Dos de Octubre, 18320 Santa Fé, Granada, Spain. Phone: 34 958441264. Fax: 34 958510712. E-mail: immunology{at}vircell.com.

Journal of Clinical Microbiology, August 2005, p. 4022-4025, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4022-4025.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.