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Journal of Clinical Microbiology, August 2005, p. 4041-4045, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4041-4045.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Biochemical Differentiation and Comparison of Desulfovibrio Species and Other Phenotypically Similar Genera

R. M. Alden Research Laboratory, Santa Monica, California 90404,¹ UCLA School of Medicine, Los Angeles, California 90095²

Received 28 January 2005/ Returned for modification 5 April 2005/ Accepted 24 May 2005

Seventeen human clinical isolates representing four species of Desulfovibrio were characterized using 16S rRNA gene sequences and tests for catalase, indole, nitrate, bile, urease, formate-fumarate stimulation, desulfoviridin, motility, and hydrogen sulfide production, plus susceptibility to antimicrobial agents. Eighty additional strains representing 10 phenotypically similar genera (Bilophila, Selenomonas, Capnocytophaga, Campylobacter, Bacteroides, Sutterella, Anaerobiospirillum, Dialister, Veillonella, and Mobiluncus) were included for comparison. All Desulfovibrio species produced H₂S and were desulfoviridin positive, and all Desulfovibrio species except D. piger were motile. The four Desulfovibrio species could be distinguished from each other using tests for catalase, indole, nitrate, urease, and growth on bile, with the following results (positive [+], negative [–], growth [G], and no growth [NG]): for D. piger, –, –, –, –, and G, respectively; for D. fairfieldensis, +, –, +, –, and G, respectively; for D. desulfuricans, –, –, +, +, and NG, respectively; and for D. vulgaris, –, +, –, –, and G, respectively. Resistance to the 10-µg colistin disk separated the Desulfovibrio species from most of the other genera, which were usually susceptible. These simple tests were useful for characterizing the Desulfovibrio species and differentiating them from other phenotypically similar genera.

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