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Pilot Study To Evaluate Microarray Hybridization as a Tool for Salmonella enterica Serovar Typhimurium Strain Differentiation

Institute of Microbiology, ETH Zürich, Wolfgang-Pauli-Str. 10, 8093 Zurich, Switzerland,¹ Robert Koch Institut, 38855 Wernigerode, Germany,² MicroDiscovery GmbH, Marienburger Strasse 1, 10405 Berlin, Germany³

Received 5 January 2005/ Returned for modification 15 March 2005/ Accepted 14 April 2005

In developed countries, Salmonella enterica subspecies 1 serovars Enteritidis and Typhimurium range among the most common causes of bacterial food-borne infections. The surveillance and typing of epidemic Salmonella strains are important tools in epidemiology. Usually, Salmonella enterica subspecies 1 serovars are differentiated by serotyping for diagnostic purposes. Further differentiation is done by phage typing as well as molecular typing techniques. Here we have designed and evaluated a prototype DNA microarray as a tool for serovar Typhimurium strain differentiation. It harbors 83 serovar Typhimurium probes obtained by differential subtractive hybridization and from the public database. The microarray yielded reproducible hybridization patterns in repeated hybridizations with chromosomal DNA of the same strain and could differentiate five serovar Typhimurium reference strains (DT204, DT104, DT208, DT36, and LT2). Furthermore, the microarray identified two distinct groups among 13 serovar Typhimurium DT104 strains. This correlated with observations from pulsed-field gel electrophoresis analysis. Twenty-three further serovar Typhimurium strains were analyzed to explore future directions for optimization of the simple 83-probe DNA microarray. The data presented here demonstrate that DNA microarrays harboring small numbers of selected probes are promising tools for serovar Typhimurium strain typing.

Supplemental material for this article may be found at http://jcm.asm.org.

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