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Journal of Clinical Microbiology, August 2005, p. 4129-4138, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4129-4138.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Prolonged Outbreak of Infection Due to TEM-21-Producing Strains of Pseudomonas aeruginosa and Enterobacteria in a Nursing Home

Véronique Dubois,1* Corinne Arpin,1 Patrick Noury,2 Catherine Andre,1 Laure Coulange,1 and Claudine Quentin1

Laboratoire de Microbiologie, Faculté de Pharmacie, EA 525, Université de Bordeaux 2, Bordeaux, France,1 Laboratoire d'Analyses Médicales, Villenave d'Ornon, France2

Received 3 March 2005/ Returned for modification 23 March 2005/ Accepted 12 April 2005

Over a 6-year period, 24 extended-spectrum ß-lactamase (ESBL)-producing isolates of Pseudomonas aeruginosa were collected from 18 patients living in a nursing home. These isolates had a delayed development of a red pigment and exhibited a similar antibiotype (resistance to all ß-lactams except for imipenem and to gentamicin, tobramycin, netilmicin, ciprofloxacin, and rifampin) associated with the production of the TEM-21 ß-lactamase and a type II 3'-N-aminoglycoside acetyltransferase [AAC(3)-II] enzyme. Surprisingly, serotyping showed that these isolates belonged to four successive serotypes (P2, P16, P1, and PME), although molecular typing by PCR methods and pulsed-field gel electrophoresis yielded identical or similar profiles. Moreover, in all isolates the blaTEM-21 gene was part of a chromosomally located Tn801 transposon truncated by an IS6100 element inserted within the resolvase gene, and the aac(3)-II gene was adjacent to this structure. During the same period, 17 ESBL-producing isolates of enterobacteria were also collected from 10 of these patients. These isolates harbored a similar large plasmid that contained the blaTEM-21 and the aac(3)-II genes and that conferred additional resistance to sulfonamides and chloramphenicol, as well as to kanamycin, tobramycin, netilmicin, and amikacin, conveyed by an AAC(6')-I enzyme. The blaTEM-21 gene was part of the Tn801 transposon disrupted by IS4321. Thus, a single clone of P. aeruginosa that had undergone a progressive genetic drift associated with a change in serotype appeared to be responsible for an outbreak of nosocomial infections in a nursing home. This strain has probably acquired the blaTEM-21-encoding plasmid that was epidemic among the enterobacteria at the institution, followed by chromosomal integration and genomic reorganization.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Faculté de Pharmacie, Université de Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. Phone: 33 5 57 57 10 75. Fax: 33 5 56 90 90 72. E-mail: veronique.dubois{at}bacterio.u-bordeaux2.fr.

Journal of Clinical Microbiology, August 2005, p. 4129-4138, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4129-4138.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dubois, V., Arpin, C., Dupart, V., Scavelli, A., Coulange, L., Andre, C., Fischer, I., Grobost, F., Brochet, J.-P., Lagrange, I., Dutilh, B., Jullin, J., Noury, P., Larribet, G., Quentin, C. (2008). {beta}-Lactam and aminoglycoside resistance rates and mechanisms among Pseudomonas aeruginosa in French general practice (community and private healthcare centres). J Antimicrob Chemother 62: 316-323 [Abstract] [Full Text]  
  • Arpin, C., Coulange, L., Dubois, V., Andre, C., Fischer, I., Fourmaux, S., Grobost, F., Jullin, J., Dutilh, B., Couture, J.-F., Noury, P., Lagrange, I., Ducastaing, A., Doermann, H.-P., Quentin, C. (2007). Extended-Spectrum-{beta}-Lactamase-Producing Enterobacteriaceae Strains in Various Types of Private Health Care Centers. Antimicrob. Agents Chemother. 51: 3440-3444 [Abstract] [Full Text]  
  • Dubois, V., Parizano, M.-P., Arpin, C., Coulange, L., Bezian, M.-C., Quentin, C. (2007). High Genetic Stability of Integrons in Clinical Isolates of Shigella spp. of Worldwide Origin. Antimicrob. Agents Chemother. 51: 1333-1340 [Abstract] [Full Text]  
  • Dubois, V., Arpin, C., Coulange, L., Andre, C., Noury, P., Quentin, C. (2006). TEM-21 extended-spectrum {beta}-lactamase in a clinical isolate of Alcaligenes faecalis from a nursing home. J Antimicrob Chemother 57: 368-369 [Full Text]  


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