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Journal of Clinical Microbiology, August 2005, p. 4163-4167, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4163-4167.2005

Inhibitor-Based Methods for Detection of Plasmid-Mediated AmpC ß-Lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis

Philip E. Coudron*

Pathology and Laboratory Medicine Service/113, McGuire Veterans Affairs, Medical Center, Richmond, Virginia 23249-0001

Received 11 February 2005/ Returned for modification 29 March 2005/ Accepted 6 May 2005

Non-beta-lactam inhibitor-based methods were evaluated for detecting plasmid-mediated AmpC ß-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis. Using CLSI methodology and disks containing cefotetan alone and in combination with 400 µg of boronic acid, 9 of 10 positive control strains and 54 of 55 AmpC-PCR-positive clinical isolates were detected. Importantly 71% and 40% of these clinical isolates were susceptible by routine testing to ceftriaxone and ceftazidime, respectively. Boronic acid disks also enhanced detection of expanded-spectrum ß-lactamases in AmpC producers.

* Mailing address: Pathology and Laboratory Medicine Service/113, McGuire Veterans Affairs Medical Center, Richmond, VA 23249-0001, Phone: (804) 675-5809. Fax: (804) 675-5518. E-mail: Philip.Coudron{at}med.va.gov.

Journal of Clinical Microbiology, August 2005, p. 4163-4167, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4163-4167.2005




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