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Journal of Clinical Microbiology, August 2005, p. 4168-4171, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4168-4171.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of ß-Lactamase Inhibitors in Disk Tests for Detection of Plasmid-Mediated AmpC ß-Lactamases in Well-Characterized Clinical Strains of Klebsiella spp.

Jennifer A. Black,1 Kenneth S. Thomson,1 John D. Buynak,2 and Johann D. D. Pitout3*

Center for Research in Anti-infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska,1 Southern Methodist University, Dallas, Texas,2 Division of Microbiology, Calgary Laboratory Services, and Departments of Pathology and Laboratory Medicine, Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada3

Received 18 February 2005/ Returned for modification 6 May 2005/ Accepted 16 May 2005

The diagnostic utility of the AmpC ß-lactamase inhibitors LN-2-128, 48-1220, and Syn 2190 in combination with cefotetan (CTT) or cefoxitin in a disk test for the detection of clinical isolates of Klebsiella spp. producing plasmid-mediated AmpC ß-lactamases (pAmpCs) was evaluated. The combination of Syn 2190 and CTT had a sensitivity of 91%, a specificity of 100%, and a reproducibility of 100% and showed the best potential of using an inhibitor for detection of Klebsiella spp. producing pAmpCs.

* Corresponding author. Mailing address: Calgary Laboratory Services, #9 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8. Phone: (403) 770-3309. Fax: (403) 770-3347. E-mail: johann.pitout{at}cls.ab.ca.

Journal of Clinical Microbiology, August 2005, p. 4168-4171, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4168-4171.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Ruppe, E., Bidet, P., Verdet, C., Arlet, G., Bingen, E. (2006). First Detection of the Ambler Class C 1 AmpC {beta}-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test. J. Clin. Microbiol. 44: 4204-4207 [Abstract] [Full Text]  
  • Jacoby, G. A., Walsh, K. E., Walker, V. J. (2006). Identification of Extended-Spectrum, AmpC, and Carbapenem- Hydrolyzing {beta}-Lactamases in Escherichia coli and Klebsiella pneumoniae by Disk Tests.. J. Clin. Microbiol. 44: 1971-1976 [Abstract] [Full Text]  


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