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Journal of Clinical Microbiology, August 2005, p. 4178-4182, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4178-4182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates

Laure Diancourt,1 Virginie Passet,1 Jan Verhoef,2 Patrick A. D. Grimont,1 and Sylvain Brisse1*

Unité Biodiversité des Bactéries Pathogènes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France,1 Eijkman-Winkler Center, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands2

Received 8 March 2005/ Returned for modification 6 May 2005/ Accepted 16 May 2005

A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.

* Corresponding author. Mailing address: Unité Biodiversité des Bactéries Pathogènes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France. Phone: 33 (0) 1 40 61 33 57. Fax: 33 (0) 1 45 68 88 37. E-mail: sbrisse{at}pasteur.fr.

Journal of Clinical Microbiology, August 2005, p. 4178-4182, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4178-4182.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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