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Journal of Clinical Microbiology, August 2005, p. 4197-4199, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4197-4199.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Approaches to Bacterial RNA Isolation and Purification for Microarray Analysis of Escherichia coli K1 Interaction with Human Brain Microvascular Endothelial Cells

Francescopaolo Di Cello, Yi Xie, Maneesh Paul-Satyaseela, and Kwang Sik Kim*

Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Park 256, Baltimore, MD 21287

Received 5 March 2005/ Accepted 10 May 2005

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in the gene expression pattern during the RNA isolation procedure.

* Corresponding author. Mailing address: Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Park 256, Baltimore, MD 21287. Phone: (410) 614-3917. Fax: (410) 614-1491. E-mail: kwangkim{at}jhmi.edu.

Journal of Clinical Microbiology, August 2005, p. 4197-4199, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4197-4199.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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