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Journal of Clinical Microbiology, August 2005, p. 4200-4202, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4200-4202.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Affordable, Abbreviated Roche Monitor Assay for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma

Susan A. Fiscus,1* Shu Chen,2 Donald Hoover,3 Melissa G. Kerkau,1 Paul Alabanza,1 Som Siharath,1 John Schmitz,1 Newton Kumwenda,2 and Taha E. Taha2

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina,1 Bloomberg School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland,2 Department of Statistics and Biostatistics, Rutgers University, New Brunswick, New Jersey3

Received 14 December 2004/ Accepted 22 April 2005

The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: fiscussa{at}med.unc.edu.

Journal of Clinical Microbiology, August 2005, p. 4200-4202, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4200-4202.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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