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Journal of Clinical Microbiology, August 2005, p. 4203-4204, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4203-4204.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Isolation and Molecular Typing of Naegleria fowleri from the Brain of a Cow That Died of Primary Amebic Meningoencephalitis

Govinda S. Visvesvara,1* Johan F. De Jonckheere,2,{dagger} Rama Sriram,1 and Barbara Daft3

Division of Parasitic Diseases, Centers for Diseases Control and Prevention, Atlanta, Georgia,1 Scientific Institute of Public Health, 1050 Brussels, Belgium,2 University of California, Davis, School of Veterinary Medicine, California Animal Health and Food Safety Laboratory System, San Bernardino Branch, San Bernardino, California3

Received 5 April 2005/ Returned for modification 16 May 2005/ Accepted 26 May 2005

Naegleria fowleri causes an acute and rapidly fatal central nervous system infection called primary amebic meningoencephalitis (PAM) in healthy children and young adults. We describe here the identification of N. fowleri isolated from the brain of one of several cows that died of PAM based on sequencing of the internal transcribed spacers, including the 5.8S rRNA genes.

* Corresponding author. Mailing address: Division of Parasitic Diseases, MS-F-13, Chamblee Campus, Centers for Diseases Control and Prevention, Atlanta, GA 30341-3724. Phone: (770) 488-4417. Fax: (770) 488-4253. E-mail: gsv1{at}cdc.gov.

{dagger} Present address: Research unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology, B1200 Brussels, Belgium.

Journal of Clinical Microbiology, August 2005, p. 4203-4204, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4203-4204.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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