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Journal of Clinical Microbiology, August 2005, p. 4229-4233, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4229-4233.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Monoclonal Antibody Routinely Used To Identify Avirulent Strains of Newcastle Disease Virus Binds to an Epitope at the Carboxy Terminus of the Hemagglutinin-Neuraminidase Protein and Recognizes Individual Mesogenic and Velogenic Strains

Judith G. Alamares, Jianrong Li,{dagger} and Ronald M. Iorio*

Department of Molecular Genetics and Microbiology, Program in Immunology and Virology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 23 August 2004/ Returned for modification 13 October 2004/ Accepted 2 May 2005

Newcastle disease virus (NDV) strains are classified as having high (velogenic), intermediate (mesogenic), or low (lentogenic) pathogenesis and virulence in chickens. Recent studies have established that the hemagglutinin-neuraminidase (HN) protein plays an important role in viral tropism and virulence. A monoclonal antibody (AVS-I) has previously been shown to be specific for lentogenic strains of NDV (Srinivasappa et al., Avian Dis. 30:562-567, 1986) and is routinely used to identify these strains. We have used competition antibody binding assays with a previously characterized panel of monoclonal antibodies, binding to chimeric HN proteins, and the characterization of an escape mutant to localize the binding site of AVS-I to the extreme carboxy terminus of the protein. In addition, we have shown that AVS-I does recognize at least one mesogenic strain and one velogenic strain of the virus, calling into question the potential of this antibody as a diagnostic reagent for avirulent NDV strains.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave. No., Worcester, MA 01655-0122. Phone: (508) 856-5257. Fax: (508) 856-5920. E-mail: ronald.iorio{at}umassmed.edu.

{dagger} Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Journal of Clinical Microbiology, August 2005, p. 4229-4233, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4229-4233.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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