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Journal of Clinical Microbiology, August 2005, p. 4251-4254, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4251-4254.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Dynamic Range and Reproducibility of Hepatitis B Virus (HBV) DNA Detection and Quantification by Cobas Taqman HBV, a Real-Time Semiautomated Assay

Magnus Lindh* and Charles Hannoun

Department of Clinical Virology, Göteborg University, Göteborg, Sweden

Received 3 March 2005/ Returned for modification 22 April 2005/ Accepted 19 May 2005

The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at ≥1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r2 = 0.97).

* Corresponding author. Mailing address: Department of Clinical Virology, Guldhedsgatan 10B, 413 46 Göteborg, Sweden. Phone: 46 0 31 342 4976. Fax: 46 0 31 827032. E-mail: magnus.lindh{at}microbio.gu.se.

Journal of Clinical Microbiology, August 2005, p. 4251-4254, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4251-4254.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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