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Journal of Clinical Microbiology, August 2005, p. 4255-4257, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4255-4257.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Primers for Clinical Detection of Paracoccidioides brasiliensis

Gioconda San-Blas,1* Gustavo Niño-Vega,1 Laura Barreto,1 Flavia Hebeler-Barbosa,2 Eduardo Bagagli,2 Rosa Olivero de Briceño,3 and Rinaldo Poncio Mendes4

Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela,1 Instituto de Biociências, UNESP, Botucatu, São Paulo, Brazil,2 Escuela de Medicina, Universidad de Carabobo, Valencia, Venezuela,3 Faculdade de Medicina, UNESP, Botucatu, São Paulo, Brazil4

Received 9 March 2005/ Returned for modification 22 April 2005/ Accepted 4 May 2005

From a 0.72-kb fragment universally generated in Paracoccidioides brasiliensis strains, primers were designed and tested on genomic DNA of this and other pathogenic fungi. They were specific and highly sensitive for P. brasiliensis DNA. Positive results were obtained when these were tested in clinical samples.

* Corresponding author. Mailing address: Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela. Phone: 58-212-504 1496. Fax: 58-212-504 1382. E-mail: sanblasg{at}ivic.ve.

Journal of Clinical Microbiology, August 2005, p. 4255-4257, Vol. 43, No. 8
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.8.4255-4257.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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