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Journal of Clinical Microbiology, September 2005, p. 4391-4401, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4391-4401.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic Analysis of Noroviruses in Chiba Prefecture, Japan, between 1999 and 2004

Mineyuki Okada,* Tomoko Ogawa, Ikuo Kaiho, and Kuniko Shinozaki

Division of Virology, Chiba Prefectural Institute of Public Health, Chiba, 260-8715, Japan

Received 27 January 2005/ Returned for modification 9 March 2005/ Accepted 31 May 2005

Noroviruses (NVs) are common pathogens that consist of genetically divergent viruses that induce gastroenteritis in humans and animals. Between September 1999 and June 2004, 1,898 samples obtained from patients showing sporadic or outbreak gastroenteritis in Chiba Prefecture, Japan, were tested for NVs by reverse transcription-PCR. NVs were detected in 603 samples. Approximately 80% were positive for genogroup GII, 13% were positive for genogroup GI, and the remaining 7% were positive for both genogroups. Phylogenetic analysis showed that the GI and GII genogroups could be further divided into 13 and 16 genotypes (including new genotypes), respectively. The GII-4 genotype, which included five small genetic clusters (subtypes), was the most common in this study and was detected in approximately 40% of positive samples. The P2 regions of 10 strains belonging to each of the five GII-4 subtypes showed 5 to 18% amino acid diversity. The amino acid substitutions accumulated in the protruding (P) region during the 5-year study period. Our data suggest that highly variable NV strains are circulating in Chiba Prefecture, with a high rate of genetic change observed during the 5-year study period.

* Corresponding author. Mailing address: Division of Virology, Chiba Prefectural Institute of Public Health, 666-2, Nitona-cho, Chuo-ku, Chiba-shi, Chiba 260-8715, Japan. Phone: 81-43-266-6723. Fax: 81-43-265-5544. E-mail: m.okd4{at}mc.pref.chiba.jp.

Journal of Clinical Microbiology, September 2005, p. 4391-4401, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4391-4401.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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