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Journal of Clinical Microbiology, September 2005, p. 4522-4527, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4522-4527.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

"Bacteroides goldsteinii sp. nov." Isolated from Clinical Specimens of Human Intestinal Origin

Yuli Song,^1* Chengxu Liu,¹ Julia Lee,¹ Mauricio Bolaos,¹ Marja-Liisa Vaisanen,¹ and Sydney M. Finegold^2,3,4

Research Service, VA Medical Center West Los Angeles, Los Angeles, California,¹ Infectious Diseases Section, VA Medical Center West Los Angeles, Los Angeles, California,² Department of Medicine, UCLA School of Medicine, Los Angeles, California,³ Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, California⁴

Received 29 March 2005/ Returned for modification 1 June 2005/ Accepted 24 June 2005

Phenotypic and phylogenetic studies were performed on an unknown gram-negative, strictly anaerobic, rod-shaped bacterium isolated from human clinical specimens. This organism was indole negative, resistant to 20% bile, produced acetic and a lesser amount of succinic acids as the major end products of glucose metabolism, and possessed a G+C content of approximately 43 mol%. Comparative 16S rRNA gene sequencing demonstrated that the unidentified bacterium was a member of the Cytophaga-Flavobacter-Bacteroides phylum of gram-negative bacteria and formed a close association (with an average sequence similarity of 93.6%) with the second subcluster of the Porphyromonas cluster in the Bacteroides subgroup. Phylogenetically and phenotypically it resembled Bacteroides merdae; however, a 16S rRNA gene sequence divergence of approximately 5.5% between the unknown bacterium and B. merdae, as well as distinguishable biochemical characteristics, demonstrate that the unknown bacterium is genotypically and phenotypically distinct and represents a previously unknown subline within the Porphyromonas phylogenetic cluster. Furthermore, a DNA-DNA reassociation value of 17.8% between isolates WAL 12034^T (the type strain of this novel taxon) and ATCC 43184^T (B. merdae type strain) also documented the separateness of the unknown species and B. merdae. Based on the phenotypic and phylogenetic findings, a new species, "Bacteroides goldsteinii sp. nov," is proposed. The G+C content of the DNA is 43 mol% for Bacteroides. The type strain of "B. goldsteinii" is WAL 12034^T (= CCUG 48944^T = ATCC BAA-1180^T).

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