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Journal of Clinical Microbiology, September 2005, p. 4580-4584, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4580-4584.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Depletion of Resident Chlamydia pneumoniae through Leukoreduction by Filtration of Blood for Transfusion

Hideaki Ikejima,1 Herman Friedman,1 German F. Leparc,2 and Yoshimasa Yamamoto1,3*

Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, Florida 33612,1 Florida Blood Services, St. Petersburg, Florida 33742,2 Department of Basic Laboratory Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan3

Received 23 August 2004/ Returned for modification 20 November 2004/ Accepted 26 May 2005

Current studies indicate that a significant percentage of healthy blood donors carry Chlamydia pneumoniae in their blood. Although the clinical significance of such findings is unknown, eradication of such bacteria from blood components may contribute to transfusion safety. Deletion of C. pneumoniae in Red Blood Cell (RBC) units was accomplished through leukoreduction by filtration. The presence of bacteria in RBC units before and after leukoreduction was assessed by real-time PCR using primers specific for C. pneumoniae 16S rRNA. The eluates of filters used for leukoreduction were also assessed by PCR and immunostaining with fluorescein isothiocyanate-conjugated chlamydial monoclonal antibodies specific for C. pneumoniae determination. Nineteen of 30 RBC units tested showed the presence of C. pneumoniae DNA. Leukofiltration resulted in a marked reduction of leukocytes as well as C. pneumoniae in terms of bacterial number and positive rate for the bacteria. The eluates of filters showed trapped bacteria determined by both PCR and immunostaining assays. Thus, leukoreduction with a filter is an effective method to significantly reduce resident C. pneumoniae levels in RBC components but may not be completely sufficient for total eradication of this pathogen.

* Corresponding author. Mailing address: Department of Basic Laboratory Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-2580. Fax: 81-6-6879-2499. E-mail: yyamamot{at}sahs.med.osaka-u.ac.jp.

Journal of Clinical Microbiology, September 2005, p. 4580-4584, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4580-4584.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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