This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sinsimer, D.
Right arrow Articles by Kreiswirth, B. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sinsimer, D.
Right arrow Articles by Kreiswirth, B. N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2005, p. 4585-4591, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4585-4591.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of a Multiplex Molecular Beacon Platform for Rapid Detection of Methicillin and Vancomycin Resistance in Staphylococcus aureus

Daniel Sinsimer,1,2 Surbhi Leekha,1 Steven Park,1 Salvatore A. E. Marras,1 Larry Koreen,1 Barbara Willey,3 Steve Naidich,4 Kimberlee A. Musser,5 and Barry N. Kreiswirth1*

Public Health Research Institute Tuberculosis Center,1 Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, International Center for Public Health, 225 Warren St., Newark, New Jersey 07103,2 Mt. Sinai Hospital, Toronto, Canada,3 Oxer Technologies, New York,4 Wadsworth Center, New York State Department of Health, Albany, New York5

Received 26 December 2004/ Returned for modification 31 March 2005/ Accepted 17 May 2005

Drug resistance, particularly vancomycin and methicillin resistance, in Staphylococcus aureus continues to emerge as a significant public health threat in both the hospital and community settings. In addition to the limited treatment options, S. aureus strains acquire and express numerous virulence factors that continue to increase its ability to cause a wide spectrum of human disease. As a result, empirical treatment decisions are confounded and there is a heightened need for a diagnostic test (or assay) to rapidly identify antibiotic resistance and specific virulence determinants and indicate the appropriate treatment. To that end we developed a platform using multiplex molecular beacon probes with real-time PCR for the rapid detection of drug resistance-determining genes and virulence factors in S. aureus. In this study, we demonstrate the specificity and sensitivity of our platform for detection of the genes conferring methicillin (mecA) and vancomycin (vanA) resistance as well as a gene encoding the virulence factor Panton-Valentine leucocidin (lukF) in S. aureus isolates.

* Corresponding author: Mailing address: PHRI TB Center, International Center for Public Health, 225 Warren St., Newark, NJ 07103. Phone: (973) 854-3240. Fax: (973) 854-3241. E-mail: barry{at}phri.org.

Journal of Clinical Microbiology, September 2005, p. 4585-4591, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4585-4591.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pingle, M. R., Granger, K., Feinberg, P., Shatsky, R., Sterling, B., Rundell, M., Spitzer, E., Larone, D., Golightly, L., Barany, F. (2007). Multiplexed Identification of Blood-Borne Bacterial Pathogens by Use of a Novel 16S rRNA Gene PCR-Ligase Detection Reaction-Capillary Electrophoresis Assay. J. Clin. Microbiol. 45: 1927-1935 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»