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Journal of Clinical Microbiology, September 2005, p. 4674-4679, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4674-4679.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Two Probes for Testing Susceptibilities of Pathogenic Yeasts to Voriconazole, Itraconazole, and Caspofungin by Flow Cytometry

Cidália Pina-Vaz,1,2* Sofia Costa-de-Oliveira,1,2 Acácio G. Rodrigues,1,2 and Ana Espinel-Ingroff3

Department of Microbiology, Porto School of Medicine,1 Institute of Pathology and Molecular Immunology, University of Porto, 4200 Porto, Portugal,2 Virginia Commonwealth University Medical Center, Richmond, Virginia 232983

Received 14 February 2005/ Returned for modification 12 April 2005/ Accepted 29 April 2005

A cytometric approach to determine the susceptibilities of Candida spp. and Cryptococcus neoformans to voriconazole, itraconazole, and caspofungin is described. A total of 63 clinical isolates with different susceptibility patterns were exposed for 1, 2, 4, and 6 h to serial concentrations of each antifungal agent, followed by staining with two fluorescent probes: propidium iodide (PI) and FUN-1. FUN-1 was able to identify the susceptibility patterns of the assayed strains to the three agents after 1 h. PI penetrated a maximum of 50% of the cells treated with PI, at the highest concentration of caspofungin, 16 µg/ml, after 6 h of incubation (this percentage varied with the strain and was drug concentration and time of incubation dependent) and did not stain cells treated with high concentrations of either azole after 6 h. The use of FUN-1 appears to be an excellent fast and reliable alternative to the classical dilution method for determining the susceptibility of Candida spp. and C. neoformans to these three antifungal agents.

* Corresponding author. Mailing address: Department of Microbiology, Porto School of Medicine, Alameda Prof. Hernani Monteiro, 4200-Porto, Portugal. Phone: 00351-919078512. Fax: 00351-229962096. E-mail: cpinavaz{at}yahoo.com.

Journal of Clinical Microbiology, September 2005, p. 4674-4679, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4674-4679.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Barbosa, J., Rodrigues, A. G., Pina-Vaz, C. (2009). Cytometric Approach for Detection of Encephalitozoon intestinalis, an Emergent Agent. CVI 16: 1021-1024 [Abstract] [Full Text]  
  • Coen, M., Bodkin, J., Power, D., Bubb, W. A., Himmelreich, U., Kuchel, P. W., Sorrell, T. C. (2006). Antifungal Effects on Metabolite Profiles of Medically Important Yeast Species Measured by Nuclear Magnetic Resonance Spectroscopy. Antimicrob. Agents Chemother. 50: 4018-4026 [Abstract] [Full Text]  


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