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Journal of Clinical Microbiology, September 2005, p. 4737-4743, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4737-4743.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Intercontinental Bovine Staphylococcus aureus Isolates

E. M. Smith,1,{dagger} L. E. Green,1* G. F. Medley,1 H. E. Bird,1 L. K. Fox,2 Y. H. Schukken,3 J. V. Kruze,4 A. J. Bradley,5 R. N. Zadoks,3 and C. G. Dowson1

Department of Biological Sciences, The University of Warwick, Coventry CV4 7AL, United Kingdom,1 Department of Veterinary Clinical Science, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-6610,2 Quality Milk Production Services, 22 Thornwood Drive, Ithaca, New York 14850,3 Microbiology Department, Faculty of Sciences, Austral University of Chile, P.O. Box 167, Valdivia, Chile,4 Division of Food Animal Science, School of Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, United Kingdom5

Received 31 December 2004/ Returned for modification 13 March 2005/ Accepted 17 April 2005

A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the differentiation of bovine S. aureus isolates from various sites (milk, teat skin, milking machine unit liners, hands, and bedding) and countries. The theory of the host specificity of S. aureus is supported by the detection of a previously undescribed clonal complex that comprised 87.4% of the isolates studied, with representatives from all geographic locations investigated. This suggests that a single clonal group has achieved a widespread distribution and is responsible for the majority of infections. Some sequence types (STs; ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05) associated with milk or teat skin.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Phone: 44 (0) 24 7652 3797. Fax: 44 (0) 24 7652 4619. E-mail: laura.green{at}warwick.ac.uk.

{dagger} Present address: Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226.

Journal of Clinical Microbiology, September 2005, p. 4737-4743, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4737-4743.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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