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Journal of Clinical Microbiology, September 2005, p. 4868-4871, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4868-4871.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Three Different PCR Methods for Quantifying Human Papillomavirus Type 16 DNA in Cervical Scrape Specimens

A. T. Hesselink,1 A. J. C. van den Brule,3 Z. M. A. Groothuismink,1 M. Molano,1 J. Berkhof,2 C. J. L. M. Meijer,1 and P. J. F. Snijders1*

Departments of Pathology,1 Biostatistics and Epidemiology, VU University Medical Center, Amsterdam, The Netherlands,2 Pathology Laboratory, Stichting PAMM, Eindhoven, The Netherlands3

Received 9 April 2005/ Returned for modification 16 May 2005/ Accepted 11 June 2005

We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

* Corresponding author. Mailing address: Dept. of Pathology, VUmc, de Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. Phone: 31-20-4443852. Fax: 31-20-4442964. E-mail: pjf.snijders{at}vumc.nl.

Journal of Clinical Microbiology, September 2005, p. 4868-4871, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4868-4871.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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