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Journal of Clinical Microbiology, September 2005, p. 4889-4890, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4889-4890.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Paired Serum Samples for Serodiagnosis of Typhoid Fever

Deborah House,1,3* Nguyen T. Chinh,6 To S. Diep,5 Christopher M. Parry,3,7 John Wain,3,4 Gordon Dougan,4 Nicholas J. White,2,3 Tran Tinh Hien,5 and Jeremy J. Farrar2,3

Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, SW7 2AZ, United Kingdom,1 Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, United Kingdom,2 Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam,3 The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom,4 Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam,5 Department of Infectious Diseases, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam,6 Department of Medical Microbiology, University of Liverpool, Liverpool, L69 3GA, United Kingdom7

Received 9 February 2005/ Returned for modification 24 March 2005/ Accepted 3 June 2005

Using an enzyme-linked immunosorbent assay we demonstrate that, in adult patients with typhoid fever, the sensitivity of a serological test based on the detection of anti-lipopolysaccharide immunoglobulin G is increased when used with paired serum samples taken 1 week apart.

* Corresponding author. Mailing address: Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, United Kingdom. Phone: 44 020 7594 3070. Fax: 44 020 7594 3069. E-mail: d.house{at}ic.ac.uk.

Journal of Clinical Microbiology, September 2005, p. 4889-4890, Vol. 43, No. 9
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.9.4889-4890.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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