This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loens, K.
Right arrow Articles by Ieven, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loens, K.
Right arrow Articles by Ieven, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2006, p. 166-171, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.166-171.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence-Based Amplification and Reverse Transcription-PCR, in Children with Acute Respiratory Infections during a Winter Season

K. Loens,1* H. Goossens,1 C. de Laat,2 H. Foolen,2 P. Oudshoorn,2 S. Pattyn,1 P. Sillekens,2 and M. Ieven1

Department of Medical Microbiology, University of Antwerp, Antwerp, Belgium,1 bioMérieux, Boxtel, The Netherlands2

Received 18 July 2005/ Returned for modification 15 September 2005/ Accepted 12 October 2005

Five hundred seventeen consecutive nasopharyngeal aspirates were collected between October 1998 and May 1999 for episodes of acute respiratory tract infections in children presenting at the University Hospital of Antwerp. Culture and nucleic acid amplification techniques—nucleic acid sequence-based amplification (NASBA) and reverse transcription-PCR (RT-PCR)—were applied to detect rhinoviruses (RVs). Other respiratory viruses were detected by immunofluorescence (IF) analysis of the specimens and IF analysis of shell vial cultures. Among the 517 specimens, 219 viral agents were identified. They were, in decreasing order, rhinoviruses (93 [18.0%]), respiratory syncytial virus (76 [14.7%]), adenoviruses (16 [3.1%]), influenza viruses (15 [2.9%]), enteroviruses (15 [2.9%]), and herpes simplex virus (4 [0.8%]). For the evaluation of rhinovirus detection, culture positivity and/or a positive reaction in the two independent amplification methods was used as an expanded "gold standard." Based on this standard, the sensitivity, specificity, positive predictive value, and negative predictive value of culture were 44.7, 100, 100, and 99.8%, and those of NASBA and RT-PCR were 85.1, 98.3, 83.3, and 98.5% and 82.9, 93.4, 55.7, and 98.2%, respectively. NASBA and RT-PCR produced comparable results and were significantly more sensitive than virus culture. RVs showed the highest incidence in acute respiratory tract infections in children.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Antwerp, Universiteitsplein 1 S3, B-2610 Wilrijk, Belgium. Phone: 32-3-820-25-51. Fax: 32-3-820-26-63. E-mail:katherine.loens{at}ua.ac.be.

Journal of Clinical Microbiology, January 2006, p. 166-171, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.166-171.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mahony, J. B. (2008). Detection of Respiratory Viruses by Molecular Methods. Clin. Microbiol. Rev. 21: 716-747 [Abstract] [Full Text]  
  • Camps Serra, M., Cervera, C., Pumarola, T., Moreno, A., Perello, R., Torres, A., Jimenez de Anta, M. T., Marcos, M. A. (2008). Virological diagnosis in community-acquired pneumonia in immunocompromised patients. Eur Respir J 31: 618-624 [Abstract] [Full Text]  
  • Lu, X., Holloway, B., Dare, R. K., Kuypers, J., Yagi, S., Williams, J. V., Hall, C. B., Erdman, D. D. (2008). Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. J. Clin. Microbiol. 46: 533-539 [Abstract] [Full Text]  
  • Wright, P. F., Deatly, A. M., Karron, R. A., Belshe, R. B., Shi, J. R., Gruber, W. C., Zhu, Y., Randolph, V. B. (2007). Comparison of Results of Detection of Rhinovirus by PCR and Viral Culture in Human Nasal Wash Specimens from Subjects with and without Clinical Symptoms of Respiratory Illness. J. Clin. Microbiol. 45: 2126-2129 [Abstract] [Full Text]  
  • Li, H., McCormac, M. A., Estes, R. W., Sefers, S. E., Dare, R. K., Chappell, J. D., Erdman, D. D., Wright, P. F., Tang, Y.-W. (2007). Simultaneous Detection and High-Throughput Identification of a Panel of RNA Viruses Causing Respiratory Tract Infections. J. Clin. Microbiol. 45: 2105-2109 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»