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Journal of Clinical Microbiology, January 2006, p. 236-238, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.236-238.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel PCR-Restriction Fragment Length Polymorphism Analysis for Rapid Typing of Staphylococcal Cassette Chromosome mec Elements

Jin Ah Yang,1,2 Dae Won Park,2,3 Jang Wook Sohn,2,3 and Min Ja Kim2,3*

Graduate School of Life Science and Biotechnology,1 Research Institute of Emerging Infectious Diseases,2 Division of Infectious Diseases, College of Medicine, Korea University, Seoul, South Korea3

Received 4 September 2005/ Returned for modification 13 September 2005/ Accepted 14 October 2005

We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Internal Medicine, Anam Hospital, Korea University College of Medicine, 126-1, Anam-dong 5th Str., Seongbuk-gu, Seoul 136-705, South Korea. Phone: (02) 920-5094. Fax: (02) 920-5616. E-mail: macropha{at}korea.ac.kr.

Journal of Clinical Microbiology, January 2006, p. 236-238, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.236-238.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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