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Journal of Clinical Microbiology, January 2006, p. 244-250, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.244-250.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Diagnostic Oligonucleotide Microarray Fingerprinting of Bacillus Isolates

Argonne National Laboratory, Argonne, Illinois 60439,¹ Pacific Northwest National Laboratory, Richland, Washington 99352,² Brigham Young University, Provo, Utah 84602³

Received 9 May 2005/ Returned for modification 3 July 2005/ Accepted 19 October 2005

A genome-independent microarray and new statistical techniques were used to genotype Bacillus strains and quantitatively compare DNA fingerprints with the known taxonomy of the genus. A synthetic DNA standard was used to understand process level variability and lead to recommended standard operating procedures for microbial forensics and clinical diagnostics.

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